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Two or more organisms that differ by a only single allele.
Isogenic strains may also be referred to in terms of different alleles being found within a common genetic background. The use of isogenic strains is to be encouraged because it makes determination of the relative fitness of individual alleles more straightforward.
An important contribution of modern molecular techniques is the potential to create isogenic strains via in vitro mutagenesis as well as the potential to check for isogenicity via whole or partial genomic sequencing.
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