Lawn

∞ generated and posted on 2016.02.20 ∞

Relatively homogeneous, turbid overgrowth of cells (particularly bacterial) growing on or near approximately the entire surface of the solid media found within a Petri dish.

Lawns are used for growing bacteriophage plaques or with the disk-diffusion method of determining antibiotic sensitivity.

One method of generation of a bacterial lawn

Figure legend: Here a bacterial culture is spread, using a cotton swab, onto the surface of a plate.

In the soft agar overlay method, which is commonly used for growing phage plaques, the bacterial lawn is grown within a thin layer of preinoculated soft or top agar that has been poured over a layer of hard or bottom agar. Alternatively. lawns can be inoculated onto the surface of hard agar using, for example, a cotton-tipped swab.


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