∞ generated and posted on 2016.01.14 ∞
Tagging of antibodies so that they glow in bright colors when bathed in UV light.
Immunofluorescence is used as a means of detecting the location in samples especially of specific proteins during microscopy.
The antibody provides specificity for binding to specific antigens while fluorescent tags provide illumination of the specific locations to which the antibodies have bound. This allows fine grained localization of especially proteins within cells and tissues.
Immunofluorescence is used particularly within the context of fluorescence microscopy, confocal microscopy, and fluorescent in situ hybridization.