Isolation of propagated recombinant DNA in vitro such as to modify that DNA or to place it in an alternative vector, in either case prior to further propagation of the modified DNA.
Cloned DNA can be shortened, combined with other DNA, moved from a cloning vector to an expression vector, etc. The point is that one starts with already cloned DNA and therefore substantial quantities of that DNA prior to refining what it is that has been cloned along with what it is that the DNA has been cloned into.
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