Iris Diaphragm

∞ generated and posted on 2016.03.18 ∞

Device that controls the amount of light that exits the condenser of a microscope, thereby controlling the illumination of the specimen and, more relevantly, the degree of contrast between specimen and background.

In light microscopy the iris diaphragm controls the size of the opening between the specimen and condenser, through which light passes. Closing the iris diaphragm will reduce the amount of illumination of the specimen but increases the amount of contrast.

The pathway of light through a compound microscope is: light sourcecondenseriris diaphragmstage → object/specimen → objective lensocular lenseye or camera.

Iris Diaphragm

Figure legend: The iris diaphragm is found within the condenser, here labeled 'Abbe condenser'. It is responsible for controlling the amount of light that passes from the condenser to the specimen or, more specifically, the width of the light beam. Narrower widths provide greater contrast but also less light. The 'trick' is to effectively balance these therefore conflicting desirables as you adjust the degree to which the iris diaphragm is opened.

If the iris diaphragm is closed too far then the specimen will be too dark to clearly view. If it is too far open then the specimen will appear to be "washed out" and perhaps also barely visible. An all too common error is to not sufficiently close the iris diaphragm and thereby fail to see the specimen due to insufficient contrast, even if the specimen otherwise is in focus. As magnification is increased, particularly as top magnification is attempted using the oil immersion lens, then increased illumination often is required, necessitating additional opening of the iris diaphragm.